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RECORD NUMBER: 2 OF 4

OLS Field Name OLS Field Data
Main Title In vitro Competition of Natural 'Autographa Californica' Nuclear Polyhedrosis Virus and a Recombinant Expressing a Polyhedrin-Beta Galactosidase Fusion Protein.
Author Kawanishi, C. Y. ; Huang, Y. S. ; Bobseine, K. L. ; Setzer, W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher Nov 88
Year Published 1988
Report Number EPA/600/D-88/259;
Stock Number PB89-129084
Additional Subjects Polyhedrosis viruses ; Recombinant proteins ; Genetic intervention ; Selection(Genetics) ; Genetic recombination ; Genetic vectors ; Autographa californica
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB89-129084 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/14/1989
Collation 9p
Abstract
The paper describes results of experiments conducted to investigate the kinetics of in vitro competition between natural progenitor Autographa californica (Acv E-2) and the recombinant Ac360-Bgal virus strains. The following conclusions can be drawn from the analysis: Selection pressure appeared to be fairly constant throughout the experiment; Selection was virtually unidirectional in favor of the natural strain regardless of the starting ratio, and consequently, the natural strain eventually predominated; The observed high frequency of mixed phenotype plaques appear to be influenced by some combinational phenomenon other than recombination at the DNA level; and The frequency of mixed phenotype plaques are also affected by passage. All evidence in the experiment points toward a disfavored recombinant genotype which appears not to be capable of competing effectively in vitro. The data present information that can be utilized as a basis for assessing the potential health effects of baculovirus expression vectors.