Three reports were generated as products of the synthesis of historical biological data project of the Tampa Bay National Estuary Program (TBNEP); 'Distribution of Selected Fish Species in Tampa Bay', 'Oligohaline Areas in Tampa Bay Tributaries: Spatial Extent and Species Lists', and 'Database of Benthic Sampling Locations in Tampa Bay'. To reduce the costs of future analyses, a benthic sampling location (BSL) computer database was developed that contains sampling locations for benthic field studies by latitude and longitude, along with information on when data were collected and what parameters were recorded. A map of all sampling locations in the database was generated by geographic information system software and submitted to TBNEP as a product of this project. The BSL database can be used to quickly identify and evaluate studies that have collected benthic samples at a given time or location. Its records are cross-referenced to the bibliographic database (BDB) developed by the Southwest Florida Water Management District.