Abstract |
The article presents an improved DNA-based embryo toxicity test in which growth is measured as the increase in DNA during a 5-h postfertilization period. The test, using direct fluorometric determination of DNA rather than thymidine incorporation, is fast, sensitive, easy to conduct, and correlates well with other 48- and 96-h acute aquatic tests. The need for radioisotopes has been eliminated and data variability is substantially reduced. It is recommended that this test be incorporated into a battery of rapid tests used to estimate aquatic toxicity. |