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RECORD NUMBER: 36 OF 86

OLS Field Name OLS Field Data
Main Title High Resolution Immunogold Localization of 'Giardia' Cyst Wall Antigens Using Field Emission SEM with Secondary and Backscatter Electron Imaging.
Author Erlandsen, S. L. ; Bemrick, W. J. ; Schupp, D. E. ; Shields, J. M. ; Jarroll., E. J. ;
CORP Author Minnesota Univ., Minneapolis. ;Cleveland State Univ., OH. ;Cleveland State Univ., OH. Dept. of Biology. ;Wisconsin Univ.-Madison.;Environmental Monitoring Systems Lab., Cincinnati, OH.
Publisher c1990
Year Published 1990
Report Number EPA-R-814622; EPA/600/J-90/096;
Stock Number PB90-244518
Additional Subjects Giardia ; Parasitology ; Cysts ; Antigens ; Reprints ; Immunohistochemistry ; Scanning electron microscopy ; Monoclonal antibodies ; Western blotting ; Polyacrylamide gel electrophoresis
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB90-244518 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/03/1990
Collation 10p
Abstract
The report describes the ultrastructural localization of Giardia cyst antigens in the filaments associated with the outer portion of intact cysts and on developing cyst wall filaments in encysting trophozoites. Postembedding immunogold labeling of thin sections of intact Giardia cysts with polyclonal and monoclonal antibodies specific for cyst wall antigens (major protein bands of approximately 29, 75, 88, and 102 kilodaltons on Western blots) showed strong labeling of the filamentous cyst wall, while no labeling was seen on the membranous portion. High resolution field emission scanning electron microscopy (FESEM) of Giardia cysts revealed that the cyst wall specific polyclonal rabbit antisera and monoclonal mouse antibody produced gold labeling of 20 nm filaments in the cyst wall as detected with secondary electron imaging (SEI) and backscatter electron imaging (BEI) at 10 kV, despite coating of the cells with platinum by ion sputtering. FESEM studies of encysting Giardia trophozoites demonstrated that immunostaining with antibodies to cyst wall antigens produced colloidal gold labeling of developing cyst wall filaments on the cell surface; however, the intervening membrane domains were unlabeled. (Copyright (c) 1990 by The Histochemical Society, Inc.)