||DNA (Deoxyribonucleic Acid) Adsorption to Soils and Sediments.
Ogram, A. ;
Sayler, G. S. ;
Gustin, D. ;
Lewis, R. J. ;
||Tennessee Univ., Knoxville.;Environmental Research Lab., Gulf Breeze, FL.;National Institutes of Health, Bethesda, MD.
Deoxyribonucleic acids ;
Soil chemistry ;
Ionic strength ;
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Deoxyribonucleic acid (DNA) adsorption of five soils, an acid-washed sand, and a lake sediment was investigated. All DNA at environmentally relevant concentrations was adsorbed by soils containing a significant amount of montmorillonite at low to neutral pH values. Studies on the effects of DNA molecular size on adsorption to sand and a sandy soil were described by the Freundlich isotherm model (r2 >0.85), and revealed that the higher the molecular weight, the more the adsorption. The effects of ionic strength (as sodium phosphate buffer) on adsorption showed that adsorption decreases as PO4 increases. Organic carbon was found to play a relatively minor role in the adsorption of DNA to these soils. A scheme for the extraction of DNA from soils was also developed. (Copyright 1988 American Chemical Society.)