||DNA-Benzo(a)Pyrene Adducts Formed in a 'Salmonella typbimurium' Mutagenesis Assay System.
Santella, Regina M. ;
Grunberger, Dezider ;
Weinstein, I. Bernard ;
||Columbia Univ., New York. Coll. of Physicians and Surgeons.;Health Effects Research Lab., Research Triangle Park, NC.;National Cancer Inst., Bethesda, MD.
||R-805482 ;PHS-CA-13696; EPA-600/J-79-147;
Deoxyribonucleic acid ;
Salmonella typhimurium ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The DNA adducts formed in Salmonella typhimurium when bacteria are incubated with radioactive benzo(a)pyrene and liver microsomal enzymes from several sources has been investigated. When enzyme prepartions from Aroclor 1254 or 3-methylcholanthrene induced C57BL/6N (B6) mice were used to mediate activation, the predominant product was n adduct between the 10 position of 7B,8a-dihydroxy-9a,10a epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene and the N-2 position of deoxyguanosine. Similar results were obtained with human liver and with Aroclor-induced rat-liver enzyme preparations.