||Correlation Between Benzo(a)pyrene-Induced Mutagenicity and DNA Adduct Formation in 'Salmonella typhimurium' TA100.
Arce, G. T. ;
Grunberger, D. ;
||Columbia Univ., New York. Coll. of Physicians and Surgeons.;Health Effects Research Lab., Research Triangle Park, NC.
Deoxyribonucleic acids ;
Salmonella typhimurium ;
Aromatic polycyclic hydrocarbons ;
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In an attempt to stabilize the dose response in the Salmonella typhimurium test (STT), the use of DNA-bound products from BP was evaluated as a measure of the biologically effective dose. In addition to the previously documented interlaboratory variation, the authors observed a 3-fold difference in the dose response of TA100 to BP even when the assay was repeated with the same experimental conditions. When overall BP-DNA adduct formation was related to the level of His+ revertants, a series of responses emerged with two predominating. In the first type of response around 70 revertants per plate were generated for every BP molecule bound per 10 to the 6th power nucleotides of cellular DNA. The second response gave about 1400 revertants per plate for one BP bound in every 10 to the 6th power nucleotides. Several intermediates curves were also detected. The variation in the mutational response to binding levels occurred regardless of the source of S9 or the growth stage of the cells. (Copyright (c) 1983 Elsevier Science Publishers B.V.)