||Comparison of Ames 'Salmonella Typhimurium' Plate Incorporation Test Protocols.
Toney, Stephanie ;
Claxton, Larry D. ;
||Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Culture media ;
Ames tests ;
Salmonella typhimurium ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The purpose of this document is to tabulate the differences between the submitted protocols and to note the modifications from the 1975 Ames reference. The tables show the differences obtained between laboratories in bacterial strain maintenance, metabolic activation systems, chemical controls, preparation of medias and solutions, and of the assay procedure itself. The recommendations of de Serres and Shelby and any revisions by Ames are also included. No attempt is made to evaluate any laboratory or indicate a preferable method for the test. Some laboratories may have Standard Operating Procedures that are more detailed than the submitted protocols. Changes or revisions that have taken place in a protocol since submission are not noted in this document. In addition, laboratories that use Ames' reference (1975) as their main protocol may have only sent their modifications.