||Basis of the Insensitivity of 'Salmonella typhimurium' Strain TA98/1,8-DNP6 to the Mutagenic Action of Nitroarenes.
McCoy, E. C. ;
Anders, M. ;
Rosenkranz, H. S. ;
||Case Western Reserve Univ., Cleveland, OH.;Health Effects Research Lab., Research Triangle Park, NC.
Salmonella typhimurium ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Salmonella typhimurium tester strain TA98/1,8-DNP6 is resistant to the mutagenicity of 1,8-dinitropyrene because it lacks an esterification enzyme which is needed for the formation of the ultimate mutagen, presumably the corresponding hydroxamic acid ester. This enzyme does not appear to be required for the activation of all nitroarenes and arylamines, as some of these are fully active in TA98/1,8-DNP. It is suggested that these form electrophilic arylnitrenium ions non-enzymatically from nitroso- and N-hydroxylamino-arenes intermediates. The esterification enzyme appears to be a transacetylase. An assay using 2-aminofluorene as the acetyl acceptor is described. Derivatives of S. typhimurium TA100 also lacking this enzyme were obtained by Tn5-mediated mutagenesis.