Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Assessment of Hazardous Wastes for Genotoxicity.
Author DeMarini, D. M. ; Houk, V. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/D-87/285;
Stock Number PB88-106984
Additional Subjects Hazardous wastes ; Toxicology ; Bioassay ; Screening ; Genotoxicity ; Salmonella typhimurium ; Mutagenesis
Library Call Number Additional Info Location Last
NTIS  PB88-106984 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 14p
The authors have evaluated a group of short-term bioassays to identify those that may be suitable for screening large numbers of diverse hazardous industrial wastes for genotoxicity. Fifteen wastes (and dichloromethane extracts of these wastes) from a variety of manufacturing processes were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100 with and without Aroclor 1254-induced rat-liver S9. Ten of these wastes were fed by gavage to F-344 male rats, and the raw urines were assayed for mutagenicity in the presence of beta-glucuronidase in strain TA98 with S9. Six of these urines were extracted by C18/methanol elution, incubated with beta-glucuronidase, and evaluated in strain TA98 with S9 and beta-glucuronidase. Fourteen of the wastes were examined for their ability to induce prophage lambda in Escherichia coli in a microsuspension assay. A second set of wastes, consisting of four industrial wastes, were evaluated in Salmonella and in a series of mammalian cell assays to measure mutagenicity, cytogenetic effects, and transformation.