Record Display for the EPA National Library Catalog

RECORD NUMBER: 551 OF 1204

OLS Field Name OLS Field Data
Main Title Potential for Transduction of Plasmids in a Natural Freshwater Environment: Effect of Plasmid Donor Concentration and a Natural Microbial Community on Transduction in 'Pseudomonas aeruginosa'.
Author Saye, D. J. ; Ogunseitan, O. ; Sayler, G. S. ; Miller, R. V. ;
CORP Author Stritch School of Medicine, Maywood, IL. Dept. of Biochemistry and Biophysics. ;Tennessee Univ., Knoxville. Dept. of Microbiology.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA/600/J-87/145;
Stock Number PB88-153820
Additional Subjects Pseudomonas ; Fresh water biology ; Reservoirs ; Deoxyribonucleic acids ; Concentration(Composition) ; Genetics ; Genes ; Bacteria ; Culture media ; Environmental tests ; Experimental data ; Models ; Graphs(Charts) ; Reprints ; Habitats ; Pseudomonas aeruginosa ; Transduction ; Plasmids
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB88-153820 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 11p
Abstract
The transduction of Pseudomonas aeruginosa plasmid Rms149 by the generalized transducing phage DS1 was shown to occur during a nine day incubation of environmental test chambers in a freshwater reservoir. Plasmid DNA was transferred from a nonlysogenic plasmid donor to a DS1 lysogen of Pseudomonas aeruginosa that served both as the source of the transducing phage and as the recipient of the plasmid DNA. Transduction of the plasmid in the presence of the natural microbial community of the reservoir was below the limits of detection employed. The results demonstrate that a potential exists for the transduction of plasmid DNA in aquatic habitats. (Copyright (c) 1987, American Society for Microbiology.)