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RECORD NUMBER: 221 OF 1202

OLS Field Name OLS Field Data
Main Title Effect of Well-Drilling Fluids of the Physiological Status and Microbial Infection of the Reef Building Coral 'Montastrea annularis'.
Author Parker, J. H. ; Nickels, J. S. ; Martz, R. F. ; Gehron, M. J. ; Richards, N. L. ;
CORP Author Florida State Univ., Tallahassee. Center for Biomedical and Toxicological Research.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1984
Report Number EPA-R-806143; EPA/600/J-84/236;
Stock Number PB85-170132
Additional Subjects Toxicity ; Drilling fluids ; Coral ; Gas wells ; Oil reservoirs ; Animal physiology ; Exposure ; Sea water ; Lipids ; Chloroform ; Methyl alcohol ; Chromatographic analysis ; Amino acids ; Bacteria ; Animal diseases ; Reprints ; Montastrea annularis ; Water pollution effects(Animals)
Holdings
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Status
NTIS  PB85-170132 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 8p
Abstract
The reef building coral Montastrea annularus was exposed continuously to suspensions of oil and gas-well drilling fluids at concentrations of 0.1 ml/liter, 0.01 ml/liter, and 0.0001 ml/liter in flowing seawater at the U.S. Naval Stage I platform (30 deg 7.5 min N, 85 deg 46.3 min W). After 6 weeks exposure, coral fragments of 12 to 65 sq cm surface area were broken off, rinsed in seawater, and extracted in a one-phase chloroform-methanol-buffer and returned to the laboratory. In the laboratory, the extraction was completed and the phases separated. The lipids were fractionated using silicic acid and thin layer chromatography. Total phospholipid, triglyceride glycerol, total extractable fatty acids, triglyceride fatty acids as well as the wax ester fatty alcohols showed no consistent changes with exposure to the drilling fluids. (Copyright (c) 1984 Springer-Veriag New York Inc.)