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RECORD NUMBER: 7 OF 10

OLS Field Name OLS Field Data
Main Title Overview, Conclusions, and Recommendations of the IPCS Collaborative Study on Complex Mixtures.
Author Claxton, L. D. ; Douglas, G. ; Krewski, D. ; Lewtas, J. ; Matsushita, H. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Health and Welfare Canada, Ottawa (Ontario). ;National Inst. of Public Health, Tokyo (Japan). ;Pittsburgh Univ., PA.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/054;
Stock Number PB92-150689
Additional Subjects Mutagens ; Toxic substances ; Mixtures ; Bioassay ; Salmonella typhimurium ; Reproducibility of results ; Interlaboratory comparisons ; SOS response(Genetics) ; Mutagenicity tests ; Metabolic activation ; Reprints ; Standard Reference Materials
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB92-150689 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 21p
Abstract
The International Programme on Chemical Safety (IPCS) and the U.S. EPA sponsored an international collaborative study to examine the variability associated with the extraction and bioassay of Standard Reference Materials (SRMs) that are complex environmental mixtures provided by the U.S. National Institute of Standards and Technology (NIST). The study evaluated the feasibility of establishing bioassay reference values and ranges for the SRMs. Twenty laboratories from North America, Europe, and Japan participated in the study. The bioassay used was the Salmonella/microsomal plate incorporation assay. Repeatability (intralaboratory variance) of the bioassay results ranged from 16% to 88% depending on the SRM and the bioassay conditions (tester strain and metabolic activation); whereas, reproducibility (interlaboratory variance) ranged from 33% to 152%. Between laboratory variability was the main source of variation accounting for approximately 55% to 95% of the total variation for the three environmental samples. Variation in the mutagenic potency of the control compounds was comparable, although the reproducibility of 1-nitropyrene ranged from 127% to 132%.