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RECORD NUMBER: 6 OF 10

OLS Field Name OLS Field Data
Main Title Optimization of the Hamilton-Thorn Computerized Sperm Motility Analysis System for Use with Rat Spermatozoa in Toxicological Studies.
Author Slott, V. L. ; Suarez, J. D. ; Poss, P. M. ; Linder, R. E. ; Strader, L. F. ;
CORP Author ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Publisher c1993
Year Published 1993
Report Number EPA-68-D2-0056; EPA/600/J-94/068;
Stock Number PB94-141603
Additional Subjects Sperm motility ; Toxicology ; Spermatozoa ; Computer simulation ; Rats ; Reproducibility of results ; Reproduction(Biology) ; Risk assessment ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB94-141603 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/14/1994
Collation 12p
Abstract
To optimize the Hamilton-Thorn Motility Analyzer (HTM; Hamilton-Thorn Research, Beverly, MA) for use in reproductive toxicology studies with rat spermatozoa, the accuracy and precision of the instrument were assessed under a variety of instrument settings. Videotapes of both fast- and slow-swimming sperm were analyzed repeatedly to obtain data across a range of sperm velocities as might be encountered as a consequence of exposure to reproductive toxicants. Acquisition rates were varied across the HTM menu choices as were the number of frames analyzed at each framing rate. For fast-swimming samples (mean straight-line velocity (VSL) approximately 130 micrometers/sec) generally good agreement between computer-assisted sperm analysis (CASA) and manually obtained data was found for percentage of motile sperm and straight-line velocity; i.e., CASA values were within 10% of manual values for most frame/rate combinations. (Copyright (c) 1993 by the Society of Toxicology.)