||Conjugal Transfer of R68.45 and FP5 between 'Pseudomonas aeruginosa' Strains in a Freshwater Environment.
O'Morchoe, S. B. ;
Oguseitan, O. ;
Sayler, G. S. ;
Miller, R. V. ;
||Stritch School of Medicine, Maywood, IL. ;Tennessee Univ., Knoxville.;Environmental Research Lab., Gulf Breeze, FL.
Fresh water biology ;
Field tests ;
Aquatic microbiology ;
Environmental persistence ;
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Recent concern over the release of genetically engineered organisms has resulted in a need for information about the potential for gene transfer in the environment. The report examines the potential for conjugal transfer of the plasmids R68.45 and FP5 by conjugation in a freshwater environment using Pseudomonas aeruginosa as a test system and Fort Loudoun Reservoir, Knoxville, Tennessee as the field site. When genetically well-defined plasmid donor and recipient strains were introduced into test chambers suspended in Fort Loudoun Lake, transfer of both plasmids was observed. Conjugation occurred both in the presence and absence of the natural microbial community. The number of transconjugants recovered was lower when the natural community was present. Transfer of the broad-host-range plasmid R68.45 to organisms other than the introduced recipient was not observed in the chambers but was observed in laboratory simulations when an organism isolated from lakewater was used as the recipient strain. (Copyright (c) 1988, American Society for Microbiology.)