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RECORD NUMBER: 8 OF 9

OLS Field Name OLS Field Data
Main Title Mitotic Stability of Transforming DNA Is Determined by Its Chromosomal Configuration in the Fungus 'Cochliobolus heterostrophus'.
Author Keller, N. P. ; Bergstrom, G. C. ; Yoder, O. C. ;
CORP Author New York State Coll. of Agriculture and Life Sciences, Ithaca. Dept. of Plant Pathology.;Corvallis Environmental Research Lab., OR.
Publisher c1991
Year Published 1991
Report Number EPA-R-813458; EPA/600/J-91/103;
Stock Number PB91-206961
Additional Subjects Mitosis ; Deoxyribonucleic acids ; Fungi ; Fungal chromosomes ; Plasmids ; Antibiotic resistance ; Hygromycin B ; Promotor regions(Genetics) ; Bacterial genes ; Nucleic acid hybridization ; Autoradiography ; Methylation ; Reprints ; Cochliobolus heterostrophus
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NTIS  PB91-206961 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/26/1991
Collation 9p
Abstract
Cochliobolus heterostrophus was transformed with a plasmid (pH1S) containing a bacterial gene (hygB), which confers resistance to the antibiotic hygromycin B when under control of an 838-bp fragment of promoter 1 from C. heterostrophus. The plasmid integrated at either homologous (52% single copy, 33% tandemly repeated copies) or ectopic (4% single copy, 11% tandemly repeated copies) sites on different chromosomes, resulting in four distinct configurations of integrated DNA. All four configurations were highly stable during mitotic growth; virtually no loss of integrated DNA was detected after five subcultures on nonselective medium or after seven cycles of pathogenesis on maize, the normal host of this fungus. However, deletion of integrated DNA was detected after eight or more disease cycles. (Copyright (c) Springer-Verlag 1991.)