||Flow Injection Analysis of Trace Hydrogen Peroxide Using an Immobilized Enzyme Reactor (Journal Version).
Hwang, H. ;
Dasgupta, P. K. ;
||Texas Tech Univ., Lubbock. Dept. of Chemistry and Biochemistry.;Environmental Protection Agency, Research Triangle Park, NC. Atmospheric Sciences Research Lab.
Hydrogen peroxide ;
Chemical analysis ;
Chemical reactors ;
Performance evaluation ;
Atmospheric composition ;
Cation exchanging ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Sub-parts per billion (ppb) levels of aqueous hydrogen peroxide have been determined with a flow injection analysis system employing a single bead string reactor composed of horseradish peroxidase covalently bound to an aminated macroporous polymeric absorbent with glutaraldehyde and a passive cation exchange membrane reactor to alter pH. The chemistry relies on the peroxidase mediated oxidation of nonfluorescent p-hydroxyphenylacetate to its fluorescent dimer. The advantage of the system includes rapid throughout rates (40 samples/h), excellent detection limits (0.3 ppb H2O2) and large dynamic range of linear response (1 ppb - 1 ppm). However, the immobilized enzyme is not useful for the analysis of organic peroxides which act as inhibitors. (Copyright (c) Springer-Verlag, 1986.)