An overview of PCR platforms -- Homogeneous fluorescent chemistries for real-time PCR -- Reference gene validation software for improved normalization -- Data analysis software -- Performing real-time PCR -- Internal and external controls for reagent validation -- Introduction to the applications of real-time PCR -- Analysis of mRNA expression by real-time PCR -- Validation of array data -- Mutation detection by real-time PCR -- Real-time NASBA -- Application in clinical microbiology -- Diagnosis of invasive fungal infections -- Biodefence -- Real-time PCR: application to food authenticity and legislation -- Molecular haplotyping by real-time PCR. "This essential manual presents a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including: clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more. An essential book for all laboratories using PCR."--BOOK JACKET.