||PCR Primers Specific for Detection of a Rat Repetitive Sequence.
Mass, M. J. ;
Roop, B. C. ;
||Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Polymerase chain reaction ;
Nucleic acid repetitive sequences ;
Molecular cloning ;
Cultured cells ;
DNA probes ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Identification of a new oncogene is often accomplished by transfection of genomic DNA of interest into a host of a different species that can respond to the altered phenotype. We report a method for tracking and identifying potential genes of interest that are derived from rat DNA. We used polymerase chain reaction to amplify specifically the rat long-interspersed repetitive sequences in mouse cells that were transfected with rat DNA.