Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Molecular Analysis of Mutations Induced at the 'hisD3052' Allele of Salmonella by Single Chemicals and Complex Mixtures.
Author DeMarini, D. M. ; Bell, D. A. ; Levine, J. G. ; Shelton, M. L. ; Abu-Shakra., A. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;National Research Council, Washington, DC.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/218;
Stock Number PB94-163961
Additional Subjects Mutations ; Salmonella ; Toxic substances ; Environmental pollutants ; Mixtures ; Alleles ; Polymerase chain reaction ; Nucleic acid conformation ; Deoxyribonucleic acids ; Sequence deletion ; Reprint ;
Library Call Number Additional Info Location Last
NTIS  PB94-163961 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 09/01/1994
Collation 8p
More single chemicals and complex environmental mixtures have been evaluated for mutagenicity at the hisD3052 allele of Salmonella, primarily in strain TA98, than in any other mutation assay. The development of colony probe hybridization procedures and the application of the of the polymerase chain reaction and direct DNA sequencing has permitted rapid molecular access to this allele. The authors discuss these techniques and the resulting mutation spectra that have been induced by a variety of environmental mutagens and complex mixtures. A common GC or CG deletion within a hot-spot region of the sequence dominates most of the spectra. In addition to this two-base deletion, they have recovered about 200 other types of mutations within the 72-base target for reversion of the hisD3052 allele. These include a variety of deletions (as large of 35 bases), duplications (as large as 46 bases), and complex mutations involving base substitutions. The quasipalindromic nature of the target sequence and its potential to form DNA secondary structures and slippage mismatches appear to be an important basis for the mutability of this allele.