Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title 32P-Postlabeling Analysis of Aromatic DNA Adducts in Fish from Polluted Areas.
Author Dunn, B. P. ; Black, J. J. ; Maccubbin, A. ;
CORP Author British Columbia Cancer Research Center, Vancouver. Environmental Carcinogenesis Unit. ;Roswell Park Memorial Inst., Buffalo, NY.;Environmental Research Lab.-Duluth, MN.
Publisher c1987
Year Published 1987
Report Number EPA-812575; EPA/600/J-87/532;
Stock Number PB90-264938
Additional Subjects Aromatic polycyclic hydrocarbons ; Fresh water fishes ; Liver neoplasms ; Hydrolysis ; Thin layer chromatography ; Foreign technology ; DNA damage ; Water pollution effects(Animals) ; Phosphorus radioisotopes ; Benzo(a)pyrene ; High pressure liquid chromatography
Library Call Number Additional Info Location Last
NTIS  PB90-264938 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 12/03/1990
Collation 7p
Brown bullheads (Ictalurus nebulosus) were sampled from sites in the Buffalo and Detroit Rivers where fish are exposed to high levels of sediment bound polycyclic aromatic hydrocarbons, and suffer from an elevated frequency of liver cancer. DNA was isolated from the livers of these wild fish and from control specimens which were raised in clean aquariums. DNA was enzymatically digested to normal and adducted nucleotides, and hydrophobic/bulky adducts were enriched in the digests either by preparative reverse-phase high-pressure liquid chromatography, or selective nuclease P1 dephosphorylation of normal nucleotides. Aromatic DNA-carcinogen adducts were then quantitated using (32)P-postlabeling analysis. Using both adduct enrichment procedures, chromatograms derived from DNA of fish from polluted areas showed a diffuse diagonal radioactive zone not present in DNA from aquarium raised fish.