Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Toxicity of organic chemicals to embryo-larval stages of fish
Author Birge, Wesley J. ; Black, Jeffrey A. ; Bruser, Donald M.
Other Authors
Author Title of a Work
Birge, Wesley J.
Black, Jeffrey A.
Bruser, Donald M.
CORP Author Thomas Hunt Morgan School of Biological Sciences, Lexington, MA.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Publisher U.S. Environmental Protection Agency,
Year Published 1979
Report Number 68-01-4321 ; EPA-560/ 11-79-007; EPA-68-01-4321
Stock Number PB80-101637
OCLC Number 08960742
Subjects Organic compounds--Toxicology ; Organic water pollutants ; Fishes--Effect of water pollution on
Additional Subjects Toxicology ; Fishes ; Bioassary ; Larvae ; Organic compounds ; Physiological effects ; Solubility ; Volatility ; Exposure ; Concentration(Composition) ; Lethal dosage ; Embryos ; Solvents ; Toxic substances ; Water pollution effects(Animals) ; Teratogenesis
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJED  EPA 560-11-79-007 OCSPP Chemical Library/Washington,DC 12/30/1999
ELDD  EPA-560/11-79-007 NHEERL/MED Library/Duluth,MN 05/30/2003
ESAD  EPA 560-11-79-007 Region 10 Library/Seattle,WA 07/09/2004
NTIS  PB80-101637 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation viii, 69 p. : ills., charts ; 28 cm.
A continuous flow procedure was developed for evaluating effects of insoluble and volatile organics on embryo-larval stages of fish. Test compounds were selected for different combinations of solubility and volatility and included aniline, atrazine, chlorobenzene, chloroform, 2,4-dichlorophenol, 2,4-dichlorophenoxyacetic acid, dioctyl phthalate, malathion, trisodium nitrilotriacetic acid, phenol, and polychlorinated biphenyl (Capacitor 21). A closed system devoid of standing air space greatly reduced volatility as a test variable. Mechanical homogenization proved highly effective in suspending hydrophobic compounds in influent water. Continuous agitation in the test chamber and regulation of detention time further precluded the need for carrier solvents. Test results indicated good reproducibility of exposure concentrations. The most toxic compounds included Capacitor 21, chlorobenzene, 2,4-dichlorophenol, and phenol. Chlorobenzene at 90 micrograms/l produced complete lethality of trout eggs. The three other compounds gave log profit LC50's of 2 to 70 micrograms/l when trout stages were exposed in hard water, and LC1's were 0.3, 1.0, and 1.7 micrograms/l for phenol, Capacitor 21, and 2,4-dichlorophenol. Chloroform also was highly toxic to trout stages and LC1's ranged from 4.9 to 6.2 micrograms/l. When bass and goldfish stages were exposed to chlorobenzene, LC1's ranged from 8 to 33 micrograms/l. Compared to other species, trout developmental stages generally exhibited the greatest sensitivity. The LC1 values determined in embryo-larval tests compared closely with maximum acceptable toxicant concentrations developed in life-cycle studies. Most compounds produced appreciable frequencies of teratic larvae.
"June 1979." "EPA-560/11-79-007." "Contract No. 68-01-4321."