Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Histochemical and Cytophotometric Assay of Acid Stress in Freshwater Fish.
CORP Author Pennsylvania State Univ., University Park. Dept. of Biology.
Year Published 1971
Report Number EPA-18050-DXJ; 18050-DXJ-05/71;
Stock Number PB-227 571
Additional Subjects Water quality ; Fishes ; Photometry ; Bioassay ; Minnows ; Toxicology ; Pathology ; Histology ; Stress(Physiology) ; Cytology ; Trout ; Fresh water fishes ; Acidity ; Kidney ; Liver ; Respiratory system ; Indicator species ; Water pollution effects(Animals) ; Gills(Fishes)
Library Call Number Additional Info Location Last
NTIS  PB-227 571 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 123p
The feasibility of using histochemical and histopathological changes in brook trout, longnose dace and fathead minnows as bioindicators of acid pollution was investigated. Laboratory studies entailed using a gravity flow diluter system. Field studies involved using net traps in polluted streams. Exposure durations were 4-5 days and 28-30 days. Histochemical and cytophotometric analyses were made of gills, Stannius corpuscle, blood, spleen, kidney and liver. The primary mode of acid toxicant action is gill damage which results in impaired respiratory, excretory and liver functions. Short term indices of acid stress include: colloidal iron and aminosalicylic acids (PAS) staining of gills and renal Stannius corpuscles. A useful bioindicator of prolonged acid exposure is decreased azure B-RNA staining of liver cells; this assesses the extent of liver impairment and reflects a reduced tolerance of fish to other toxicants. (Modified author abstract)