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RECORD NUMBER: 4 OF 15

OLS Field Name OLS Field Data
Main Title Effects of Selected Neuroactive Chemicals on Calcium Transporting Systems in Rat Cerebellum and on Survival of Cerebellar Granule Cells.
Author Kodavanti, P. R. S. ; Mundy, W. R. ; Tilson, H. A. ; Harry, G. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Div. of Intramural Research.
Publisher 18 Jun 93
Year Published 1993
Report Number EPA/600/J-94/071;
Stock Number PB94-141595
Additional Subjects Cerebellum ; Toxic substances ; Nervous system ; Calcium ; Rats ; Biological transport ; Ca(2+)-transporting ATPase ; Cell survival ; Lactate dehydrogenase ; Aluminum ; N-methyl aspartate ; Mitochondria ; Microsomes ; Chlorpromazine ; Reprints ; Permethrin ; Deltamethrin
Holdings
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Status
NTIS  PB94-141595 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/14/1994
Collation 11p
Abstract
This investigation examined the relationship between alteration of Ca(2+)-transport systems and cytotoxicity in vitro for a number of neuroactive chemicals including environmental pollutants. (45)Ca(2+) uptake as a measure of Ca(2+) sequestration was determined in mitochondria and microsomes isolated from cerebella of adult male Long-Evans hooded rats by differential centrifugation. Ca(2+) extrusion, measured as Ca(2+)-ATPase activity, was determined in synaptosomes prepared by sucrose density gradient. Cytotoxicity (lactate dehydrogenase leakage) was assessed in primary cultures of cerebellar granule cells from 6- to 8-day old Long-Evans rats. N-Methyl-D-aspartic acid (NMDA) did not alter synaptosomal Ca(2+)-ATPase activity or (45)Ca(2+) uptake in mitochondria and microsomes. However, chlorpromazine (CPZ), aluminum (Al), permethrin (PER), and deltamethrin (DEL) inhibited Ca(2+) sequestration by mitochondria and microsomes. Of all the chemicals tested, CPZ was the most potent in inhibiting Ca(2+)-transporting systems and was also cytotoxic.