Contents Notes |
A routine procedure to effectively survey algae for potential degradative ability has been developed. The pesticidal compound is added to algae cultures in log, or active growth phase. After incubation for seven days the cultures are analyzed for metabolites. This procedure allows one to minimize the possibilities of pesticide inhibition of growth. Also, controls have been expanded to include addition of pesticide to killed algae cultures as well as to cell-free media. This is done primarily to monitor the degree of degradation due to non-metabolic processes. All pesticides are C14-labeled and degradation products examined by comparative TLC and autoradiography. The results to date of this survey are summarized. |