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RECORD NUMBER: 9 OF 9

OLS Field Name OLS Field Data
Main Title Rat Liver Subcellular Fractions Catalyze Aerobic Binding of 1-Nitro((sup 14)C)Pyrene to DNA (Deoxyribonucleic Acids).
Author Ball, L. M. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/302;
Stock Number PB86-162328
Additional Subjects Deoxyribonucleic acids ; Liver ; Rats ; Laboratory animals ; Binding ; Reprints ; Nitropyrene
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB86-162328 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 6p
Abstract
The recently characterized environmental mutagen and potential carcinogen 1-nitropyrene (NP) is known to bind to DNA in Salmonella typhimurium, and also in anaerobic incubations catalysed by purified xanthine oxidase. In the study the authors show that rat liver S9 supernatant, microsomal and cytosolic subcellular fractions are also able to catalyse the binding of 1-nitropyrene labelled with 14C to calf thymus DNA in vitro. In incubations conducted under air, S9 and microsomes from Charles River CD rats were the most active fractions, and NADPH was required for maximum activity (25-100 pmol NP bound/mg DNA/mg protein in 1 hour). S9 and microsomes had about one-fourth the activity under nitrogen, although less of the activity was NADPH-dependent. Binding in cytosolic incubations was generally low (1 to 5 pmol NP/mg DNA/mg protein in 1 hour), was somewhat enhanced under N2, and was more extensive in the absence of NADPH. Treatment of rats (Harlan Sprague Dawley) with the inducing agents phenobarbital (PB), Aroclor 1254 (A) or 3-methylcholanthrene (3-MC) enhanced NADPH-dependent binding in aerobic S9 (2 to 5-fold) and microsomal (10 to 20-fold) incubations.