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RECORD NUMBER: 46 OF 47

OLS Field Name OLS Field Data
Main Title Trophic Control of Lung Development by Sympathetic Neurons: Effects of Neonatal Sympathectomy with 6-Hydroxydopamine.
Author Slotkin, T. A. ; Lau, C. ; Kavlock, R. J. ; Whitmore, W. L. ; Queen., K. L. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC. ;Duke Univ. Medical Center, Durham, NC. Dept. of Pharmacology.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA-68-02-4450, EPA-R-813769; EPA/600/J-88/539;
Stock Number PB91-109207
Additional Subjects Nerve cells ; Lungs ; Ribonucleic acids ; Deoxyribonucleic acids ; Polyamines ; Body weight ; Reprints ; Sympathetic nervous system ; Chemical sympathectomy ; Hydroxydopamines ; Newborn animals ; Ontogeny ; Up-regulation(Physiology) ; Beta adrenergic receptors,Ornithine decarboxylase ; Cyclic AMP ; Organ weight
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB91-109207 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/04/1991
Collation 17p
Abstract
The onset of peripheral sympathetic neuronal function is thought to provide trophic regulatory signals for development of adrenergic target tissues. In the current study, the effects on lung development of neonaatal sympathectomy with 6-hydroxydopamine were examined. The completeness of the lesion and effectiveness in reducing sympathetic input to the tissue were confirmed by direct measurement of norepinephrine levels and turnover. Despite the denervation, no evidence of beta-receptor up-regulation was found; in fact, receptor binding sites tended to be reduced throughout development. The cyclic AMP response to isoproterenol challenge was initially suppressed in the lesioned animals, but became supersensitive even in the face of reduced receptor binding capabilities. Evidence was also obtained for ontogenetic abnormalities in the ornithine decarboxylase/polyamine system, which is partially controlled by beta-adrenergic input and which regulates macromolecule synthesis in replicating and differentiating cells. Eventually, the alterations were reflected in aberrant developmental patterns of DNA, RNA and protein in the lung. These results indicate that sympathetic neurons influence the biochemical development of the lung and may serve to program permanently the relationships among receptor sites, receptor coupling to cellular function, and control of cell maturation.