Record Display for the EPA National Library Catalog

RECORD NUMBER: 313 OF 1145

OLS Field Name OLS Field Data
Main Title Determination of Giardia cyst viability /
Author Meyer, Ernest A.
CORP Author Oregon Univ. Health Sciences Center, Portland. Dept. of Microbiology and Immunology.;Municipal Environmental Research Lab., Cincinnati, OH.
Publisher Municipal Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency ; Available through the National Technical Information Service,
Year Published 1979
Report Number EPA/600/2-79-063; EPA-R-804898
Stock Number PB80-108285
OCLC Number 13610144
Subjects Giardia. ; Protozoa. ; Drinking water--Oregon.
Additional Subjects Neoplasms ; Cysts ; Parasites ; Viability ; Determination ; Water ; Protozoa ; Intestines ; Humans ; Animals ; Giardia ; Infections
Internet Access
Description Access URL
http://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9101XW1J.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EHAM  QD142.M37 Region 1 Library/Boston,MA 04/29/2016
EJAD  EPA 600/2-79-063 Region 3 Library/Philadelphia, PA 06/16/2000
EJBD ARCHIVE EPA 600-2-79-063 Headquarters Library/Washington,DC 04/04/2016
EKAM  EPA-600/2-79-063 Region 4 Library/Atlanta,GA 09/24/1999
ELBD  EPA 600-2-79-063 AWBERC Library/Cincinnati,OH 06/04/1999
ERAD  EPA 600/2-79-063 Region 9 Library/San Francisco,CA 10/01/2012
ESAD  EPA 600-2-79-063 Region 10 Library/Seattle,WA 07/15/2005
NTIS  PB80-108285 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation viii, 28 pages : illustrations ; 28 cm.
Abstract
The principal objective of this research was the development of a sensitive, standardized method of determining whether or not Giardia cysts are viable. The availability of such a method is necessary to determine the effect of chemical and physical agents on the viability of these organisms in water. Using cysts collected from an asymptomatic human carrier, a method for inducing excystation was developed and optimized with regard to pH, time of acid exposure, incubation temperature and excystation medium. The method was applied in determining the effects of cyst storage in water at various temperatures on cyst survival as determined by the cultural excystation method and eosin dye exclusion. The results indicate that cyst survival time decreased with increasing temperatures above 0C. Freezing and thawing resulted in almost complete loss of viability.
Notes
Prepared for U.S. Environmental Protection Agency, Office of Research and Development, Municipal Environmental Research Laboratory, Cincinnati, Ohio, under grant no. R804898. Includes bibliographical references (pages 24-27).