||Chromosomal Changes Associated with Tumorigenic Mineral Fibers.
Palekar, L. D. ;
Eyre, J. F. ;
Coffin, D. L. ;
||Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Silicate minerals ;
Chromosome abnormalities ;
Laboratory animals ;
Pleural neoplasms ;
Cell lines ;
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The purpose of the study is to investigate the cytogenetic characteristics of mineral fiber-induced rat pleural mesotheliomas. Cell lines were established in vitro from 6 chrysotile- and 7 erionite-induced rat pleural mesotheliomas. Each cell line was composed of several cell populations that were heterogeneous in their chromosome make-up, but a predominant cell population was readily recognizable in each cell line based on chromosome number. The cell lines were characterized by either aneuploid or pseudodiploid cells with chromosome modal numbers ranging from 40-92. Karyotype analyses revealed a heterogeneous display of chromosome rearrangements. Each cell line had unique abnormalities. No recurrent abnormality could as yet be associated with either chrysotile or erionite-induced mesothelioma cells. However, frequent anomalies of chromosome No. 1 suggest chromosome No. 1 involvement in the pathogenesis of rat pleural mesotheliomas.