||Cloning, Expression, and Regulation of the 'Pseudomonas cepacia' Protocatechuate 3,4-Dioxygenase Genes.
Zylstra, G. J. ;
Olsen, R. H. ;
Ballou, D. P. ;
||Michigan Univ., Ann Arbor. Medical School.;Environmental Research Lab., Gulf Breeze, FL.
Molecular cloning ;
Gene expression ;
Protocatechuate 3-4-dioxygenase ;
Bacterial genes ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Genes for the alpha and beta subunits of the enzyme protocatechuate 3,4-dioxygenase were cloned from the Pseudomonas cepacia DB01 chromosome on a 9.5 kilobase pair PstI fragment into the broad-host-range cloning vector pR02317. The resultant clone was able to complement protocatechuate 3,4-dioxygenase mutations in P. cepacia, P. aeruginosa, and P. putida. Expression studies showed that the genes were constitutively expressed and subject to catabolite repression in the heterologous host. Since the cloned genes exhibited normal induction patterns when present in P. cepacia DB01 it was concluded that induction was subject to negative control. (Copyright (c) 1989 American Society for Microbiology).