Record Display for the EPA National Library Catalog

RECORD NUMBER: 40 OF 287

OLS Field Name OLS Field Data
Main Title Cloning and Expression of the catA and catBC Gene Clusters from 'Pseudomonas aeruginosa' PAO.
Author Kukor, J. J. ; Olsen, R. H. ; Ballou, D. P. ;
CORP Author Michigan Univ., Ann Arbor. Medical School.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1988
Year Published 1988
Report Number EPA-R-812679; EPA/600/J-88/529;
Stock Number PB90-264920
Additional Subjects Mutations ; Catalysis ; Reprints ; Pseudomonas aeruginosa ; Reiterated genes ; Molecular cloning ; Gene expression ; Plasmids ; Restriction mapping ; Transfection ; Recombination(Genetics) ; Bacterial DNA
Holdings
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Status
NTIS  PB90-264920 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/03/1990
Collation 10p
Abstract
A 9.9-kilobase (kb) BAMIII restriction endonuclease fragment encoding the catA and catBC gene clusters was selected from a gene bank of the Pseudomonas aeruginosa PAO1c chromosome. The catA, catB, and catC genes encode enzymes that catalyze consecutive reactions in the catechol branch of the beta-ketoadipate pathway. A recombinant plasmid, pRo1783, which contains the 9.9-kb BAMIII restriction fragment complemented P. aeruginosa, mutants with lesions in the catA, catB, or catC gene; however, this fragment of chromosomal DNA did not contain any other catabolic gene which had been placed near the catA or catBC cluster based on cotransducibility of the loci. Restriction mapping, deletion subcloning, and complementation analysis showed that the order of the genes on the cloned chromosomal DNA fragment is catA, catB, catC. (Copyright (c) 1988, American Society for Microbiology.)