||Litton Bionetics, Inc., Bethesda, MD.; Occidental Chemical Corp., Grand Island, NY.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
The mutagenicity of crotonaldehyde was evaluated in Salmonella tester strains TA1535, TA1537, TA1538, TA98 and TA100, both in the presence and absence of added metabolic activation by Aroclor-induced rat liver S9 fraction. Based on preliminary bacterial toxicity determinations, crotonaldehyde was tested for mutagenicity at concentrations of 0.005, 0.01, 0.10, 1.0, 5.0 and 10.0 ul/plate using the agar incorporation method. Crotonaldehyde did not produce a positive response in any bacterial tester strain, either with or without metabolic activation. The investigators reported crotonaldehyde was toxic to all bacterial strains at concentrations of 1, 5 and 10 ul/plate.