||Mutagenicity and Clastogenicity of Proflavin in L5178Y/TK(+/-)-3.7.2.C Cells.
DeMarini, D. M. ;
Brock, K. H. ;
Doerr, C. L. ;
Moore, M. M. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Chromosome abnormalities ;
Genetic mutations ;
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The paper evaluated the ability of proflavin to induce specific-locus mutations at the heterozygous thymidine kinase (tk) locus of L5178Y/TK+/- -3.7.2.C mouse lymphoma cells, which permit the recovery and classification of mutants due to single-gene or chromosomal mutation. Proflavin was highly mutagenic at the tk locus, producing 724-965 TK mutants/10(6); survivors. The potent clastogenicity of proflavin was confirmed by cytogenetic analysis for chromosomal aberrations. These results lead to the conclusion that proflavin induces few single-gene mutations in mammalian cells; instead, it is a potent clastogen and produces primarily chromosomal mutations. The authors discuss the possible involvement of DNA topoisomerase II in the clastogenic mechanism of proflavin.