Record Display for the EPA National Library Catalog

RECORD NUMBER: 102 OF 265

OLS Field Name OLS Field Data
Main Title Molecular and Cytogenetic Analysis of Lung Tumor Cell Lines.
Author Hozier, J. ;
CORP Author Florida Inst. of Tech., Melbourne.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher Mar 90
Year Published 1990
Report Number EPA-R-813944; EPA/600/1-90/004;
Stock Number PB90-183443
Additional Subjects Pulmonary neoplasms ; Cytology ; Lymphocytes ; Tables(Data) ; Cells ; Chromosomes ; Molecular structure ; Ribonucleic acids ; Deoxyribonucleic acids ; Genetic mapping ; DNA probes ; Nucleic acid hybridization ; Oncogenes ; Cultured tumor cells ; Karyotyping
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-183443 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/15/1990
Collation 53p
Abstract
The report measures the levels of amplification of oncogenes and tumor marker genes or other genes of interest in nine human lung tumor cell lines in comparison to normal human bronchial epithelial cells or normal blood lymphocytes to test the hypothesis that aberrant amplification of marker genes may be related to their proximity on the human genome to oncogenes similarly amplified during the development of malignancy. Six pairs of comapping genes and an additional unpaired oncogene were used for analysis: the oncogenes MYCL and BLYM, KRASIP and prolactin; KRASIP and chronic gonadotropin, alpha chain; MYB and chorionic gonadotropin, alpha chain; MYC and thyroglobulin; FOS and creatine kinase, brain form, and MUCN. Of 31 gene pairs tested, amplification was observed in three cases. In two out of those three, co-amplification was observed in the MYC gene and the closely mapping thyroglobulin gene.