||Repeated Sequences Including RS1100 'Pseudomonas cepacia' AC1100 Function as IS Elements.
Haugland, R. A. ;
Sangodkar, U. M. X. ;
Chakrabarty, A. M. ;
||Illinois Univ. at the Medical Center, Chicago. Dept. of Microbiology and Immunology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
Pseudomonas cepacia ;
Nucleic acid repetitive sequences ;
Bacterial DNA ;
Restriction mapping ;
Nucleic acid hybridization ;
Gene expression ;
DNA insertion elements ;
Molecular cloning ;
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Several lines of evidence were obtained that the previously identified, repeated sequence RS1100 of Pseudomonas cepacia strain AC1100 undergoes transposition events. DNA sequences flanking the chlorohydroxy hydroquinone (CHQ) degradative genes of the organism were examined from sources, including several independently isolated cosmid clones from an AC1100 genomic library and genomic DNAs of two independently maintained wild-type AC1100 isolates. Hybridization and restriction endonuclease mapping studies revealed these sequences to be similar except for their numbers and distributions of RS1100 copies. A recombinant plasmid containing the immediate chq gene region and excluding any copies of RS1100 was conjugated into AC1100 mutant RHA5 which was shown to have undergone a deletion of its corresponding DNA. Hybridization and restriction mapping analyses of several reisolated plasmids revealed the presence of RS1100 sequences at different positions within either the vector or insert portions. One such plasmid contained tandem copies of RS1100 with an intervening DNA sequence also of AC1100 origin. (Copyright (c) Springer-Verlag 1990.)