||Method 1601 : male-specific (F+) and somatic coliphage in water by two-step enrichment procedure.
||DynCorp Information and Enterprise Technology, Inc., Fairfax, VA.;Environmental Protection Agency, Washington, DC. Office of Water.
|| U.S. Environmental Protection Agency, Office of Water,
||EPA 821-R-01-030; EPA-68-C-98-139
Viral pollution of water--United States--Prevention. ;
Microbial contamination--United States--Prevention. ;
Bacteriophages--United States--Prevention. ;
Water--Pollution--Health aspects--United States--Prevention. ;
Sewage sludge ;
Quality control ;
Pollution prevention ;
Waste management ;
Data analysis ;
EPA Method 1601 ;
Waste water treatment
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||vi, 32 pages : illustrations, charts ; 28 cm
Coliphage presence in ground water is an indication of fecal contamination. Method 1601 (two-step enrichment procedure) is a performance-based method for detecting the presence of male-specific (F+) and somatic coliphage in ground water and other waters. Laboratories are permitted to modify or omit any steps or procedure, with the exception of the coliphage stock enumeration procedure (Section 11.3), provided that all performance requirements set forth in the validated method are met. The laboratory may not omit any quality control analyses. The two-step enrichment procedure requires enrichment of coliphage in a nutrient broth with host bacteria followed by spotting onto a lawn of host bacteria and assessing lysis zone formation in the lawn. This two-step enrichment method was validated as a qualitative, presence-absence method, and Method 1601 was written with this use in mind. Although the method potentially may be used as a quantitative assay of coliphage concentrations in an MPN format, the two-step enrichment method has not been validated this way. This method is for use in the Environmental Protection Agency's (EPA's) data gathering and monitoring programs under the Safe Drinking Water Act and the Clean Water Act.
Cover title. "April 2001." Includes bibliographical references.