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RECORD NUMBER: 31 OF 31

OLS Field Name OLS Field Data
Main Title Zinc Amelioration of Cadmium-Induced Teratogenesis In Vitro.
Author Warner, C. W. ; Sadler, T. W. ; Tulis, S. A. ; Smith, M. K. ;
CORP Author North Carolina Univ. at Chapel Hill. School of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA/600/J-84/135;
Stock Number PB85-124568
Additional Subjects Toxicology ; Cadmium ; Mice ; Embryos ; Laboratory animals ; Reprints ; Teratogenesis
Holdings
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NTIS  PB85-124568 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 9p
Abstract
Mouse embryos were exposed to cadmium (5.35 micrograms/ml) for either 0.5h or 1h and then transferred to fresh serum for the duration of the experiment. Embryos exposed for 0.5h developed numerous malformations that were primarily localized to the craniofacial region. Embryos exposed for 1 h to the same level of cadmium all failed to thrive. In a separate series of experiments zinc (10 micrograms/ml) was added 15 minutes prior to the addition of cadmium. Embryos treated with cadmium for 30 min. after the addition of zinc, resulted in 15 of 16 embryos developing normally. In the second group of embryos treated for 1 h after preincubation with zinc resulted in 93% of the embryos surviving after 48h, albeit malformed. These results suggest that: (1) Exposure time to a teratogen is a critical parameter to consider when designing in vitro experiments (2) zinc is effective in protecting embryos against cadmium induced teratogenesis and (3) the whole embryo culture system is effective in demonstrating biological interactions between agents.