Main Title |
Flow Injection Analysis of Trace Hydrogen Peroxide Using an Immobilized Enzyme Reactor (Journal Version). |
Author |
Hwang, H. ;
Dasgupta, P. K. ;
|
CORP Author |
Texas Tech Univ., Lubbock. Dept. of Chemistry and Biochemistry.;Environmental Protection Agency, Research Triangle Park, NC. Atmospheric Sciences Research Lab. |
Publisher |
c1986 |
Year Published |
1986 |
Report Number |
EPA-R-812366; EPA/600/J-85/538; |
Stock Number |
PB89-110464 |
Additional Subjects |
Hydrogen peroxide ;
Chemical analysis ;
Membranes ;
Enzymes ;
Chemical reactors ;
Performance evaluation ;
Fluorescence ;
Monitoring ;
Atmospheric composition ;
Cation exchanging ;
Acid precipitation
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-110464 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
14p |
Abstract |
Sub-parts per billion (ppb) levels of aqueous hydrogen peroxide have been determined with a flow injection analysis system employing a single bead string reactor composed of horseradish peroxidase covalently bound to an aminated macroporous polymeric absorbent with glutaraldehyde and a passive cation exchange membrane reactor to alter pH. The chemistry relies on the peroxidase mediated oxidation of nonfluorescent p-hydroxyphenylacetate to its fluorescent dimer. The advantage of the system includes rapid throughout rates (40 samples/h), excellent detection limits (0.3 ppb H2O2) and large dynamic range of linear response (1 ppb - 1 ppm). However, the immobilized enzyme is not useful for the analysis of organic peroxides which act as inhibitors. (Copyright (c) Springer-Verlag, 1986.) |