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RECORD NUMBER: 7 OF 215

OLS Field Name OLS Field Data
Main Title Analysis of the Mechanism of Methylmercury Cytotoxicity.
Author Massaro, E. J. ; Elstein, K. H. ; Zucker, R. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;NSI Technology Services Corp., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/D-90/042;
Stock Number PB90-221748
Additional Subjects Toxicology ; Flow cytometry ; Mitosis ; Deoxyribonucleic acids ; Methylmercury compounds ; Cell survival ; Chromosome aberrations ; Cell membrane ; Dose-response relationships
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB90-221748 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/27/1990
Collation 10p
Abstract
MeHg-induced perturbation of the cell cycle kinetics of the murine erythroleukemic cell (MELC) has been investigated by flow cytometry (FCM). It was observed that, at relatively low levels (2.5 - 7.5 microM), MeHg predominately inhibits progression through the S phase of the cell cycle (in a dose-dependent manner). Accumulation of cells in the G2/M phase of the cycle also occurs, but to a considerably lesser extent. Light microscopy reveals a dose-dependent increase in incidence of chromosomal aberrations (condensation, pulverization). Higher dose levels (10 - 50 microM), induce chromosomal ring formation and progressive perturbation of the cell membrane/cytoplasm complex. The latter is manifested as increased 90 deg light scatter (refractive index (Shapiro, 1988)), decreased axial light loss (cell size (Cambier and Monroe, 1983)), simultaneous propidium iodide and carboxyfluorescein fluorescence, and resistance to detergent (NP-40)-mediated cytolysis (Zucker et al., 1989). Observations indicate that DNA synthesis is the primary target of MeHg cytotoxicity and that apparent targets and degree of cytotoxicity are a complex function of dose.