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RECORD NUMBER: 6 OF 213

OLS Field Name OLS Field Data
Main Title Ammonia Removal from Mammalian Cell Culture Medium by Ion-Exchange Membranes.
Author Sikdar, S. K. ; Sawant, S. B. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Bombay Univ. (India). Dept. of Chemical Technology.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/371;
Stock Number PB95-124863
Additional Subjects Ammonia ; Cultured cells ; Culture media ; Ion exchange resins ; Artifical membrane ; Lactic acid ; Reprints ; Perfluorosulfonic acid polymer membranes
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB95-124863 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/06/1995
Collation 13p
Abstract
Metabolites such as ammonia and lactic acid formed during mammalian cell culture can frequently be toxic to the cells themselves beyond a threshold concentration of the metabolites. Cell culture conducted in the presence of such accumulated metabolites is therefore limited in productivity. This work demonstrates with laboratory data that a nonporous ion-exchange membrane of the perfluorinated sulfonic acid type can be used to contact the culture medium, and ammonia removed selectivity from the medium without disturbing the process. The technique of pervaporation showed particular promise in this regard. The pervaporation used with inert gas sweep on the permeate side was found superior to that used with vacuum application.