||Effects of holding time, storage, and the preservation of samples on sample integrity for the detection of fecal indicator bacteria by quantitative polymerase chain reaction (qPCR)-based assays /
Wymer, Larry, ;
L. Wymer ;
K. Oshima ;
J. Paar ;
M. Doolittle ;
||TechLaw, Inc., Bethesda, MD.; Environmental Protection Agency, Lexington, MA. Region 1.
|| U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory,
Water pollution monitoring ;
Fecal pollution ;
Membrane filters ;
Quality control ;
Enteric pathogens ;
Pollution prevention ;
Waste management ;
RNA gene sequences ;
Quantitative polymerase chain reaction (qPCR) ;
||Region 10 Library/Seattle,WA
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||1 volume (various pagings) : tables, figures ;
The purpose of this project was to answer questions related to storage of samples to be analyzed by the quantitative polymerase chain reaction (qPCR)-based assays for fecal indicator bacteria. The report is divided into two parts. The first part describes studies that were performed to determine if filters that are used to collect fecal indicator bacteria can be stored frozen and analyzed at a later date. These studies were primarily directed at a specific, targeted question: can qPCR results from freezer archived samples be used to establish valid relationships between fecal indicator densities and health effects data collected from previous epidemiological studies. The second part describes studies that were performed to determine if refrigerated water samples can be held for 24 to 48 hours prior to analysis by qPCR. These studies addressed a question that may be relevant to the implementation of the qPCR method for water quality monitoring at remote locations where immediate analysis of the samples is not possible.
"EPA/600/R-10/150." "December 2010." Includes bibliographical references.