Record Display for the EPA National Library Catalog

RECORD NUMBER: 1 OF 3897

OLS Field Name OLS Field Data
Main Title 'Spodoptera frugiperda' Nuclear Polyhedrosis Virus Genome: Physical Maps for Restriction Endonucleases BamHI and HindIII.
Author Loh, Lambert C. ; Hamm, John J. ; Huang, Eng-Shang ;
CORP Author North Carolina Univ. at Chapel Hill.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-R-806210; EPA-600/J-81-517;
Stock Number PB82-153768
Additional Subjects Genes ; Viruses ; Maps ; Microorganisms ; Genetics ; Reprints ; Spodoptera frugiperda ; Nuclear polyhedrosis viruses ; Endonucleases
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB82-153768 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 12p
Abstract
The physical map for the genome of Spodoptera frugiperda nuclear polyhedrosis virus was constructed for restriction endonucleases BamHI and HindIII. The ordering of the restriction fragments was accomplished by cross-blot hybridization of BamHI, HindIII, and EcoRI fragments. The alignment of the HindIII fragments within the BamHI map was achieved by double digestion with the two restriction endonucleases followed by cross-blot hybridization. The results showed that the viral genome consisted of mainly unique sequences. In addition, the circular nature of the viral genome was reaffirmed.