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RECORD NUMBER: 46 OF 96

OLS Field Name OLS Field Data
Main Title Heterogeneity of Human Natural Killer (NK) Cells: Enrichment of NK by Negative-Selection with the Lectin from 'Erythrina cristagalli' (Journal Version).
Author Harris, D. T. ; Iglesias, J. L. ; Argov, S. ; Toomey, J. ; Koren, H. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. ;Duke Univ. Medical Center, Durham, NC. ;Ben-Gurion Univ. of the Negev, Beersheba (Israel).
Publisher c1987
Year Published 1987
Report Number EPA/600/J-87/431;
Stock Number PB89-105878
Additional Subjects Immunology ; Lectins ; Natural killer cells ; Antibody-dependent cell cytotoxicity ; Humans ; Lymphokines ; Isolation ; Lymphocytes ; Reprints ; Erythrina cristagalli
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB89-105878 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/14/1989
Collation 9p
Abstract
A novel technique for the isolation and enrichment of human natural killer (NK) cells from peripheral blood mononuclear cells (MNC) is described. Negative selection of MNC with the lectin from Erythrina cristagalli (ECA), whether by panning or agglutination in solution, resulted in a population of lymphocytes (5-20% of original MNC) highly enriched in cells exhibiting NK function. The enrichment was evident by a significant increase (range of 3-50-fold) in cells with large granular lymphocyte (LGL) morphology, K562 tumor-binding cells, cytotoxic activity, and cells expressing NK phenotypic markers (Leu 11+, OKM1+). Analysis of the cytolytic specificity of the cells demonstrated that the lytic spectrum was typical of endogenous NK. The effector cells were responsive to augmentation of cytotoxic potential by lymphokines (IL-2, IFN-alpha, and IFN-gamma) and capable of antibody-dependent cell-mediated cytotoxicity (ADCC). ECA-negative (ECA(-)) cells were equivalent to NK isolated by Percoll gradient fractionation. NK heterogeneity was demonstrated by the observation of a small percentage (1-5% of MNC) of NK in the ECA(+) population. (Copyright (c) 1987 Alan R. Liss, Inc.)