Record Display for the EPA National Library Catalog

RECORD NUMBER: 26 OF 96

OLS Field Name OLS Field Data
Main Title Direct DNA Sequencing of PCR Products.
Author Zimmerman, L. J. ; Fuscoe, J. C. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/053;
Stock Number PB92-150671
Additional Subjects Deoxyribonucleic acids ; Polymerase chain reaction ; Hypoxanthine phosphoribosyltransferase ; Polyacrylamide gel electrophoresis ; Southern blotting ; T-lymphocytes ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB92-150671 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/28/1992
Collation 5p
Abstract
Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single- and double-stranded PCR products. (Copyright (c) 1991 Wiley-Liss, Inc.)