Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title In vitro Lymphocyte Proliferation Assays: The Mitogen-Stimulated Response and the Mixed Lymphocyte Reaction in Immunotoxicity Testing.
Author Smialowicz, R. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher 1991
Year Published 1991
Report Number EPA/600/D-91/284;
Stock Number PB92-126911
Additional Subjects Toxicity ; Lymphocytes ; Mitogens ; Cell division ; Immunology ; In vitro analysis ; Lymphocyte transformation ; Test methods ; Spleen ; Rats ; Mice ; Toxic substances ;
Library Call Number Additional Info Location Last
NTIS  PB92-126911 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 02/24/1992
Collation 27p
The report describes detailed methodologies for the determination of the in vitro mitogen-stimulated response and one-way mixed lymphocyte reaction of mouse and rat lymphocytes. A list of reagents, supplies and equipment necessary for the successful completion of these assays is provided. Also, a step-by-step description of each of these assays is presented, so that laboratories unfamiliar with these procedures should be able to perform them. These assays are useful for the identification and characterization of agents capable of altering the immune system and as such are useful as screening tests for potential immunotoxicants.