Record Display for the EPA National Library Catalog

RECORD NUMBER: 638 OF 962

OLS Field Name OLS Field Data
Main Title Induction of DNA-Protein Crosslinks in Human Cells by Ultraviolet and Visible Radiations: Action Spectrum.
Author Peak, J. G. ; Peak, M. J. ; Sikorski, R. S. ; Jones, C. A. ;
CORP Author Argonne National Lab., IL.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/264;
Stock Number PB86-158573
Additional Subjects Radiation effects ; Deoxyribonucleic acid ; Humans ; Cells(Biology) ; Ultraviolet radiation ; Light(Visible radiation) ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB86-158573 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 10p
Abstract
DNA-protein crosslinking was induced in cultured human P3 teratocarcinoma cells by irradiation with monochromatic radiation with wavelengths in the range 254-434 nm (far-UV, near-UV, and blue light). Wavelength 545 nm green light did not induce these crosslinks, using the method of alkaline elution of the DNA from membrane filters. The action spectrum for the formation of DNA-protein crosslinks revealed two maxima, one in the far-UV spectrum that closely coincided with the relative spectrum of DNA at 254 and 290 nm, and one in the visible light spectrum at 405 nm, which has no counterpart in the DNA spectrum. The primary events for the formation of DNA-protein crosslinks by such long-wavelength radiation probably involve photosensitizers. This dual mechanism for DNA-protein crosslink formation is in strong contrast to the single mechanism for pyrimidine dimer formation in DNA, which apparently has no component in the visible light spectrum.