Main Title |
Transcription factor protocols / |
Other Authors |
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Publisher |
Humana Press, |
Year Published |
2000 |
OCLC Number |
40489061 |
ISBN |
0896035735; 9780896035737 |
Subjects |
Transcription factors--Laboratory manuals ;
Transfection--méthodes ;
Analyse de séquence d'ADN--méthodes ;
Facteurs de transcription--analyse ;
Wetenschappelijke technieken ;
Séquenðcage des acides nucléiques ;
Transcription Factors--analysis--https://idnlmnihgov/mesh/D014157Q000032 ;
Sequence Analysis, DNA--methods--https://idnlmnihgov/mesh/D017422Q000379 ;
Transfection--methods--https://idnlmnihgov/mesh/D014162Q000379
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Internet Access |
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Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
ELDM |
QP552.T68T727 2000 |
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CCTE/GLTED Library/Duluth,MN |
08/11/2000 |
|
Collation |
x, 306 pages : illustrations ; 23 cm. |
Notes |
Includes bibliographical references and index. |
Contents Notes |
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis. |