Abstract |
The mutagenicity of methacrylic acid, methyl ester was evaluated in Salmonella tester strains TA98, TA100, TA1535, and TA1537 (Ames Test), both in the presence and absence of added metabolic activation by Aroclor-induced rat liver S9 fraction. Based on the results of preliminary bacterial toxicity testing, the material was tested for mutagenicity at doses of 0, 1000, 2500, 5000, 7500, and 10000 ug/plate using the direct plate incorporation method; mutagenicity was additionally tested for in strain TA100 at concentrations of 0, 10, 25, and 50 mM using the suspension assay. The material was found to be mutagenic in strain TA100, in the presence of activation, in the suspension assay. Methacrylic acid, methyl ester did not cause a reproducible positive response in tester strains TA98, TA100, TA1535, or TA1537, either in the presence or absence of added metabolic activation, in the direct plate incorporation assay. |