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RECORD NUMBER: 8 OF 8

OLS Field Name OLS Field Data
Main Title Response of Plant-Colonizing Pseudomonads to Hydrogen Peroxide.
Author Katsuwon, J. ; Anderson, A. J. ;
CORP Author Utah State Univ., Logan. Dept. of Biology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1989
Year Published 1989
Report Number EPA-R-815294; EPA/600/J-89/427;
Stock Number PB90-264847
Additional Subjects Pseudomonas ; Hydrogen peroxide ; Plants(Botany) ; Response(Biology) ; Catalase ; Reprints ; Superoxide dismutase ; Isoelectric focusing ; Isoenzymes ; Polyacrylamide gel electrophoresis
Holdings
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Status
NTIS  PB90-264847 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 12/03/1990
Collation 7p
Abstract
Colonization of plant root surfaces by Pseudomonas putida may require mechanisms that protect this bacterium against superoxide anion and hydrogen peroxide produced by the root. Catalase and superoxide dismutase may be important in this bacterial defense system. Stationary-phase cells of P. putida were not killed by hydrogen peroxide (H2O2) at concentrations up to 10 mM, and extracts from these cells possessed three isozymic bands of catalase activity in native polyacrylamide gel electrophoresis. Logarithmic-phase cells exposed directly to hydrogen peroxide concentrations above 1 mM were killed. Extracts of logarithmic-phase cells displayed only band A catalase activity. Protection against 5 mM H202 was apparent after previous exposure of the logarithmic-phase cells to nonlethal concentrations (30 to 300 microM) of H2O2. Extracts of these protected cells possessed enhanced catalase activity of band A and small amounts of bands B and C. A single form of superoxide dismutase and isoforms of catalase were apparent in extracts from a foliar intercellular pathogen, Pseudomonas syringae pv. phaseolicola. (Copyright (c) 1989, American Society for Microbiology.)