Issued also in French under title: Guide des essais de pathog�enicit�e et de toxicit�e de nouvelles substances microbiennes pour les organismes aquatiques et terrestres. Co-authors: D. McLeay ... et al. Cf. Acknowledgements. Includes bibliographical references (pages 154-164).

The intent of this document is to provide guidance on preparing for and conducting single-species tests to measure and evaluate the pathogenicity and/or toxicity of new microbial substances to aquatic and terrestrial organisms. It focuses on the information requirements with respect to laboratory tests for measuring the potential ecological effects of new microbial substances on the following six categories of test (host) organisms, that might be required by Environment Canada as part of the New Substances Notification (NSN) Regulations under the Canadian Environmental Protection Act, 1999: (1) an aquatic plant; (2) an aquatic invertebrate; (3) an aquatic vertebrate; (4) a terrestrial plant; (5) a terrestrial invertebrate; and (6) a terrestrial vertebrate. The guidance herein is intended for notifiers, environmental consultants, study directors, and principal investigators. It will assist in the selection of an appropriate series of biological test methods for measuring the pathogenicity and/or toxicity of new microbial substances, as well as in the planning, execution, and reporting phases associated with each test.